Abstract
Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd.) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 μCi of [ 3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call