Abstract
A cell line, SP-2, was established from spleen tissue ofBairdiella chrysura (the silver perch). The line is susceptible to lymphocystis virus and the amphibian LT-1 virus but refractory to six additional viruses. The modal chromosome number of primary silver perch cells is 48, but SP-2 cells are heteroploid. For growth, Leibovitz L-15 medium supplemented with fetal bovine serum and sodium chloride (to 0.150m) was employed. Cells replicated best at 25° to 28° C.
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