Abstract
An ELISA method has been developed for the determination of albumin in human parotid saliva. The range of sensitivity is 2–80 μg/1 and it is suitable for use with volumes of 5 μl or less of freshly collected parotid fluid, without prior sample preparation. Calibration data were fitted by microcomputer to an exponential function. The albumin concentration of parotid saliva was 1.9 ± 1.53 mg/l, but levels fell on sample storage. Isoelectric focusing, followed by immunoblotting with antiserum to human serum albumin, showed identical double bands of pI 4.9–5.0 in albumin from both serum and saliva, thereby confirming that the antigens from the two sources are identical.
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