Abstract
In pH 8.4 Tris-HCl buffer solutions, the alcohol dehydrogenase (ADH) catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide (NAD) to produce acetaldehyde. The acetaldehyde reduce HAuCl4 to form gold particles, and the excess HAuCl4 have enhancement effect on the chemiluminescence reaction of luminol-H2O2. The excess HAuCl4 decreased when ethanol increased, and the chemiluminescence value decreased. Accordingly we can utilize the chemiluminescence to detect the content of ethanol indirectly. The decreased chemiluminescence value (ΔI) was proportional to the ethanol concentration (C) from 0.017 to 5.10 mmol/L, with a regression equation of ΔI = 2374.3C+280.1, and a detection limit of 2.3 μmol/L. This proposed method was applied to detect ethanol in saliva samples of drunk, with satisfactory results
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