Abstract
In pH 8.4 Tris-HCl buffer solutions, the alcohol dehydrogenase (ADH) catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide (NAD) to produce acetaldehyde. The acetaldehyde reduce HAuCl4 to form gold particles, and the excess HAuCl4 have enhancement effect on the chemiluminescence reaction of luminol-H2O2. The excess HAuCl4 decreased when ethanol increased, and the chemiluminescence value decreased. Accordingly we can utilize the chemiluminescence to detect the content of ethanol indirectly. The decreased chemiluminescence value (ΔI) was proportional to the ethanol concentration (C) from 0.017 to 5.10 mmol/L, with a regression equation of ΔI = 2374.3C+280.1, and a detection limit of 2.3 μmol/L. This proposed method was applied to detect ethanol in saliva samples of drunk, with satisfactory results
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.