Abstract
In pH 8.4 Tris–HCl buffer solutions, alcohol dehydrogenase catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide to produce acetaldehyde. In the medium of HCl, acetaldehyde reduced HAuCl4 to form gold particles that exhibited a strong resonance scattering (RS) peak at 600 nm. The RS peak increased with ethanol concentration. The increased RS intensity at 600 nm (ΔI600 nm) was proportional to the ethanol concentration (C) from 0.068 to 10.2 mmol/L, with a regression equation of ΔI600 nm = 35.59 C + 16.1, and a detection limit (3σ) of 3.2 μmol/L. This proposed method was applied to detect ethanol in saliva and plant cell culture medium samples, with satisfactory results.
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