An electrophysiological demonstration of axonal projections of single ventral inspiratory neurons to the phrenic nucleus of the cat

Abstract

Axonal branching patterns of single inspiratory (I) neurons of the nucleus retroambigualis (NRA) were studied electrophysiologically in cat phrenic nucleus (C4–C6). Experiments were performed on Nembutal anesthetized, artificially ventilated cats, and extracellular spikes of I neurons were recorded. The cervical spinal gray matter was microstimulated from dorsal to ventral sites at 100 μm intervals with an intensity of 150–250 μA using a glass insulated tungsten microelectrode. The stimulations were made at 1 mm intervals rostrocaudally along the spinal cord, and effective stimulating sites of antidromic activation in axonal collaterals were systematically mapped. I neurons examined ( n = 8) descending contralaterally distributed multiple collaterals in the phrenic nucleus. These collaterals were found throughout the rostrocaudal phrenic nucleus. An I neuron ( n = 1) descending ipsilaterally also distributed collaterals in the ipsilateral phrenic nucleus. Axonal collaterals in the contralateral phrenic nucleus occupied 44.2% of the total length of the cervical spinal cord examined. To determine the detailed trajectory of collaterals in the cervical gray matter, microstimulation was performed in and around the collateral arborizations at the maximum intensity of 50 μA. The descending stem axons could be localized in the lateral funiculus in four I neurons and in the ventral funiculus in one I neuron. I neurons distributed axonal collaterals within the phrenic nucleus. Some part of the collaterals ran to the medial region of the gray matter, re-crossed the midline under the central canal and reached the phrenic nucleus ipsilateral to the I neuron. Re-crossed collaterals arborized in the phrenic nucleus, but did not extend to the gray matter more lateral than the phrenic nucleus. Rostrocaudal extension of the re-crossed collaterals was found to be narrow.