Abstract
The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed sandwich-type immunoassay shows good performance. This method establishes a feasible amperometric immunoassay method for sensitive analysis of AFP in serum samples. Under the optimal experimental conditions, the DPV current response of the immunosensor is proportional to the logarithmic value of the AFP concentration. The linear detection range can achieve to 0.005–20 ng/mL with a detection limit of 1.5 pg/mL. The proposed immunosensor has good precision, selectivity and stability, and can be used for AFP determination in clinical tests.
Highlights
Alpha-fetoprotein (AFP) is a glycoprotein, mainly produced by embryonic liver cells
Methylene blue (MB), cetyltrimethylammonium bromide (CTAB), NaBH4, and trisodium citrate were purchased from Sinopharm Chemical Co., Ltd
The diameter of the Au cubes was at an average of 80 nm, in which the structure facilitates the immobilization of anti-AFP antibody and horseradish peroxidase (HRP) enzyme (Yu et al, 2017)
Summary
Alpha-fetoprotein (AFP) is a glycoprotein, mainly produced by embryonic liver cells. About 2 weeks after birth, AFP disappears from the blood. The content of AFP in adult serum is
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