Abstract
Eggplant (Solanum melongena L.) represents the third most important crop of the Solanaceae family and is an important component of our daily diet. A population of 164 F6 recombinant inbred lines (RILs), derived from two eggplant lines differing with respect to several key agronomic traits, “305E40” and “67/3,” was grown to the commercial maturation stage, and fruits were harvested, separated into peel and flesh, and subjected to liquid chromatography Liquid Chromatography/Mass Spectrometry (LC/MS) analysis. Through a combination of untargeted and targeted metabolomics approaches, a number of metabolites belonging to the glycoalkaloid, anthocyanin, and polyamine classes and showing a differential accumulation in the two parental lines and F1 hybrid were identified. Through metabolic profiling of the RILs, we identified several metabolomic quantitative trait loci (mQTLs) associated with the accumulation of those metabolites. Each of the metabolic traits proved to be controlled by one or more quantitative trait loci (QTLs); for most of the traits, one major mQTL (phenotypic variation explained [PVE] ≥ 10%) was identified. Data on mQTL mapping and dominance–recessivity relationships of measured compounds in the parental lines and F1 hybrid, as well as an analysis of the candidate genes underlying the QTLs and of their sequence differences in the two parental lines, suggested a series of candidate genes underlying the traits under study.
Highlights
Eggplant (Solanum melongena L.) is a diploid species (2n = 2x = 24) belonging to the Solanaceae family, genus Solanum and subgenus Leptostemonum
The recombinant inbred lines (RILs) population composed of 164 F6 RILs (Toppino et al, 2020), as well as the two parental lines “305E40” (P1, female parent) and “67/3” and the F1 hybrid (HF1), were grown in an experimental field at Montanaso Lombardo (45◦20′N, 9◦26′E) in two independent, randomized blocks, each constituting a biological replicate
Peaks showing more than a 2-fold change between the two parental lines or between the F1 hybrid and either of the parental line with a p ≤ 0.05 were classified as differentially abundant (DA) and are shown in the “untargeted” section of Table 1
Summary
Eggplant (Solanum melongena L.) is a diploid species (2n = 2x = 24) belonging to the Solanaceae family, genus Solanum and subgenus Leptostemonum. It has originated in Africa and was probably domesticated in Asia (Weese and Bohs, 2010), where it has been cultivated for over 1,500 years. Eggplant fruits differ strongly in size, shape, and skin color Most of their nutritional properties are related to their content in phenolics, especially chlorogenic and hydroxycinnamic acids and their conjugates, as well as in the skin of pigmented genotypes, anthocyanins, and other phenylpropanoids (Whitaker and Stommel, 2003; Mennella et al, 2012)
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