Abstract
The introduction of a short spacer arm was necessary to obtain complete regioselectivity in the glycosylation of β-cyclodextrin (β-CD) mediated by glycosidases. Thus 6- N-(2-aminoethyl-α- d-galactopyranosyl)-6-deoxycyclomaltoheptaose was prepared in four steps from β-cyclodextrin with 30% overall yield using, in the key step, the transfer activity of green coffee bean α-galactosidase.
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