Abstract
A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledonary embryo germination. This medium was also used for EC seedlings propagation and adventitious bud induction from SN explants. Regenerated plantlets were cultured on hormone-free MS medium for elongation and root induction. The regeneration capability of SN explants was compared by using EC seedling lines established in this research. EC seedling line EL6 exhibited the highest adventitious bud induction frequency (91.7%) and the highest number of buds per responding explant (5.2), which was considered as the most efficient EC seedling line for further gene transformation research.
Highlights
Cinnamomum camphor is a large, handsome evergreen tree that grows up to 20~30 m (66~98 ft) tall
This medium could be used for embryo germination induction, propagation of EC seedlings and adventitious bud induction from SN explants of EC seedlings
The apical dominance of single cultured EC seedlings was not significant, with multiple shoots derived from the base of seedlings (Fig 2C). 2 weeks later in light culture, 17 EC seedlings with multiple shoots were obtained in this research
Summary
Cinnamomum camphor (commonly known as camphor tree or camphor laurel) is a large, handsome evergreen tree that grows up to 20~30 m (66~98 ft) tall. Extensive work has been carried out for juvenile explants supplier selection and direct organogenesis induction of camphor tree, including embryos germination induction, screening and propagation of highly proliferation embryo-cultured (EC) seedling lines, adventitious buds induction from explants, elongation and rooting of regenerated shoots. An efficient plantlet regeneration system in camphor tree via direct organogenesis was established through nodal segments from embryo-cultured seedlings, which could be used in future transformation studies.
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