Abstract
The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.
Highlights
Arachis hypogaea, an annual oilseed crop of the Fabaceae family, is native to South America but is currently grown in diverse environments around the world (Sharma and BhatnagarMathur 2006)
Studies were performed in peanut (Arachis hypogaea L.) cultivar L14 which was provided by Field Crops Research Institute, Vietnam Academy of Agricultural Sciences
The present study showed that more than 81% of sterile peanuts cultivar L14 have germinated on MS medium after 3 days of incubation (Figure 1A) and by day 7 have grown into in vitro whole plant
Summary
Arachis hypogaea (peanut, groundnut), an annual oilseed crop of the Fabaceae family, is native to South America but is currently grown in diverse environments around the world (Sharma and BhatnagarMathur 2006). Peanut is known as an important industrial and oilseed crop in many of the warmer regions of the world. Pests and diseases are two of the causes leading to poor peanut productivity (Nageshwara-Rao and Nigam 2001). The transfer of genes for resistance to pests and diseases into peanuts is considered a promising solution to increase field productivity (Dang et al 2019; Vasavirama and Kirti 2012). To transfer genes into plants, completing an efficient in vitro regeneration system for them is a very important first step. Studies have shown that the response of peanut cultivars to the culture medium is very different. It is almost difficult to use a nutrient medium for this peanut cultivar to culture the others if a high regeneration efficiency is desired
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