Abstract
Arnebia hispidissima, which belongs to the family Boraginaceae, is an important medicinal and dye yielding plant. The alkannin, a red dye, are root-specific secondary metabolites of A. hispidissima. Shoots were regenerated from callus derived from immature inflorescence explants obtained from field grown plants. MS medium containing 4.52μM 2, 4-D and 3.33μM BAP was found to be most effective for the proliferation of callus, induced on medium containing 4.52μM 2, 4-D. Maximum number (43.1 ± 0.25) with average length (5.2 ± 0.23) of shoots regenerated when callus was transferred to MS medium supplemented with 1.11μM BAP, 1.16μM Kin and 0.57μM IAA. About 75.5% of in vitro regenerated shoots were rooted on half-strength MS medium supplemented with 9.84μM of IBA and 200mg l(-1) of activated charcoal. In comparison to in vitro, higher percent (90.2%) of shoots were rooted under ex vitro conditions when treated with IBA (0.98mM) for 5min. Plantlets rooted in vitro as well as ex vitro were acclimatized successfully under the green house conditions. Ex vitro rooted plants exhibited higher survival percentage (75%) as compared to in vitro rooted plantlets (60%). Present study may be applicable in the large-scale root-specific red dye (alkannin) production via root induction under ex vitro condition.
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