Abstract
The diverse structures of nucleic acids as scaffolds have brought the significant advancement for DNA‐based enantioselective catalysis, yet RNA‐based enantioselective catalysis is lacking investigation. Herein, we report a small, natural RNA of cyclic di‐AMP (c‐di‐AMP) and Cu2+ ions assemble into an artificial metalloribozyme (c‐di‐AMP·Cu2+), that could effectively catalyze the enantioselective Diels–Alder reactions with up to 80 % ee. The enantioselective catalytic performance of c‐di‐AMP·Cu2+ has been studied by thorough investigations of different metal cofactors, c‐di‐AMP/Cu2+ molar ratios, additives, buffers and c‐di‐AMP analogues. In addition, the assembly of c‐di‐AMP·Cu2+ gives rise to 300‐fold and 5‐fold rate acceleration compared to the uncatalyzed reaction and Cu2+ ions, respectively. This work provides a simple and efficient strategy to construct the RNA‐based catalysts that would expand the current nucleic acids‐based catalysis and might hint the possible catalytic RNA in primordial chemistry.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call