Abstract
An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige and Skoog media supplemented with 3% sucrose, 0.8% agar, and various concentrations of indole-3-butyric acid (1 to 10 mg/L). Cultures were maintained for 4 to 5 weeks at standard conditions to allow root induction and elongation. Shoot induction was triggered upon altering the daytime culture room temperature to 37 ± 2 °C. Multiple shoots were produced at higher IBA concentrations. Another 5 weeks later, individual plantlets were excised and hardened for 2 weeks in soil preparation contained in small cups before transferring to 30 × 20 × 20 cm sized pots. We discuss the probable events effectuating unusual shoot regeneration at relatively higher temperatures in media without supplementing any cytokinins.
Highlights
Snake plants represent about 70 species of monocot angiosperms in the genus Sansevieria belonging to the Asparagaceae family of the order Nolinoideae
We studied the effects of a plant growth regulator Indole-3-butyric acid (IBA) (Indole 3 btyric acid) and a shift in culture room temperature on productive regeneration efficiency in Sansevieria trifasciata (ST) which catered successfully devising a rapid, easy and proficient in vitro clonal propagation protocol for this valuable plant
For root induction and maintenance, leaf segments were further excised to 1cm2 explants, each of which were aseptically transferred to glass jam jars (400 ml volumetric capacity), each containing 100 ml of autoclaved (121 0C, 20 min) MS (Murashige and Skoog, 1962) basal media (Himedia, cat# PT099) supplemented with various concentrations (1.00–10.00 mgL− 1) of Indole-3-butyric acid (IBA) (Himedia, cat# PCT0804), 0.8% agar (Himedia, cat# RM7695) and 3% sucrose (Himedia, cat# PCT0607)
Summary
Snake plants represent about 70 species of monocot angiosperms in the genus Sansevieria belonging to the Asparagaceae family of the order Nolinoideae. We studied the effects of a plant growth regulator IBA (Indole 3 btyric acid) and a shift in culture room temperature on productive regeneration efficiency in ST which catered successfully devising a rapid, easy and proficient in vitro clonal propagation protocol for this valuable plant.
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