An effective method for plasma lipoprotein separation: Studies of various animal species

Abstract

1. 1. Plasma lipoprotein separation by density gradient ultracentrifugation largely depends on visual examination based on the natural yellow pigments of lipoproteins. 2. 2. In non-human species and in humans with dyslipoproteinemia, some lipoproteins are not well visualized due to the lack of pigments. 3. 3. Using a fluorescent probe in minute quantity (1,6 diphenyl-1,3,5-hexatriene) we were able to demonstrate an effective plasma lipoprotein separation using a discontinuous density grandient ultra-centrifugation technique. 4. 4. Plasma lipoproteins of human, chicken, rat and carp were compared showing the unique character of carp HDL.