Abstract
AbstractA novel asymmetrical cyanine dye (TR) with NIR absorption at 970 nm was synthesized and applied in photoacoustic imaging (PAI) in vivo for the first time. The advantages of asymmetrical structure in PAI was demonstrated with DFT calculation and verified by related experiments. BSA was selected as the matrix to encapsulate TR‐BSA nanoparticles (TR‐NPs) which was investigated as a PAI agent. Exceptional performance of TR‐NPs was shown in Balb/c rats caudal vein model; the details could be clearly displayed and identified at a 0.1 mm scale.
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