Abstract
Escherichia coli (E. coli) is a universal host for molecular cloning of DNA, as well as for assessing clone genes. By way of background, E. coli is an anaerobic and coli-form bacteria that is often found in the lower intestine area of warm blooded animals. When transforming a bacterial organism such as E. coli, there are several key factors to consider. The first factor is the technique used to produce competence for transformation, and the second factor is the genetic makeup of the bacterial organism that is being transformed. The variety of the genes in the experiment greatly impacts the outcome of the extent of the transformation. This analysis observed E. coli treated with various elements across four different scenarios (each referred to as a distinct “Plate”). It was revealed that E. coli subjected to the conditions of Plate B (i.e., treated with +pGLO, luria broth, arabinose, and ampicillin) contained the ideal environment for transforming bacterial cells.
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