Abstract
Biofilm is an extracellular matrix produced by bacteria that provides resistance to antimicrobials. It has been proposed that biofilms play an important role in chronic bacterial endometritis, and anti-biofilm treatments with acetylcysteine and TRIS-EDTA have been recommended. However, very little is known about biofilms produced by bacteria associated with equine endometritis. Isolates of E. coli, K. pneumoniae, and P. aeruginosa from equine uterine cultures were obtained during the 2013 breeding season. Static in vitro biofilm formation was determined for each organism by culturing the bacteria in Nunc Bacti 96 well plates containing 98 ml lysogeny broth and 2 ml bacterial organism, incubated at 37 C for 24 hours, biofilm biomass was determined by staining with crystal violet and absorbance quantified using a microplate reader. Isolates were considered to be positive for a biofilm if the 95% confidence interval for the isolate did not overlap with the negative
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