An aptamerelectrochemical sensor based on functional carbon nanofibers for tetracycline determination

Abstract

Fe-Co@CNF was synthesized by electrospinning technology, and AuNPs was loaded onto Fe-Co@CNF by in-situ reduction to obtain Fe-Co@CNF@AuNPs composite material, which was used as the working electrode based on Au-S bond cooperation. The tetracycline electrochemical sensing interface Fe-Co@CNF@AuNPs@Apt was constructed by connecting mercaptoylated tetracycline (TC) aptamers on Fe-Co@CNF@AuNPs surface. The morphology and composition of Fe-Co@CNF@AuNPs composites were characterized by SEM, TEM, EDS, XRD and XPS, and the electrochemical properties of tetracycline were evaluated by CV and DPV. The results showed that the addition of Fe and Co did not destroy the structure of the original carbon nanofibers, and their synergistic effect enhanced the electrocatalytic performance, effective electrode area and electron transfer ability of carbon nanofibers. AuNPs are evenly distributed over the fibers, which effectively improves the electrical conductivity of the material. Under the optimal conditions, the theoretical detection limit of tetracycline was 0.213 nM, and the linear detection range was 5.12–10 mM, which could successfully detect tetracycline in milk.