An electrochemiluminescence sensor based on Ag NPs amplifying PDDA-modified TbPO4:Ce NWs signal for sensitive detection of lincomycin

Abstract

The residue of lincomycin in water will not only aggravate the drug resistance of bacteria but also cause damage to the human body through biological accumulation. In this work, an electrochemiluminescence (ECL) aptasensor for the detection of lincomycin was constructed based on polydimethyldiallylammonium chloride (PDDA) functionalized Ce-doped TbPO4 nanowires (PDDA-TbPO4:Ce NWs) and silver nanoparticles (Ag NPs). TbPO4:Ce NWs were used as the luminophore, and PDDA was used to functionalize the luminophore to make the surface of the luminophore positively charged. The negatively charged silver nanoparticles were combined with PDDA-TbPO4:Ce NWs by electrostatic interaction. Ag NPs accelerated the electron transfer rate and promoted the ECL efficiency, which finally increased the ECL intensity of TbPO4:Ce NWs by about 4 times. Under the optimal conditions, the detection limit of the ECL sensor was as low as 4.37 × 10−16 M, and the linear range was 1 × 10 − 15 M to 1 × 10 − 5 M, with good selectivity, stability, and repeatability. The sensor can be applied to the detection of lincomycin in water, and the recovery rate is 97.7–103.4 %, which has broad application prospects.