Abstract
A modified method for a single run separation and identification of the molecular species of different phospholipid classes in a complex extract has been proposed. It includes reverse phase HPLC with a mass spectrometric detection. This approach has been employed for the analysis of glycerophospholipids and sphingolipids of human erythrocytes and several ceramide fractions have been identified[L2], that were missed in previous studies employing similar methods. The proposed scheme of experiment decreased the number of procedures needed for a complete phospholipid profiling of the sample.
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