An ancestral NB-LRR with duplicated 3\u2032UTRs confers stripe rust resistance in wheat and barley

Chaozhong Zhang,Lin Huang,Youliang Zheng,Ming Hao,Fei Ni,Huifei Zhang,Fengjuan Chen,Meinan Wang,Juan Qin ,Bo Lyu,Jiajie Wu,Lianquan Zhang,Dengcai Liu,Lynn Epstein,C.l Kou ,Miao Liu,Ge Gao,Jirui Wang,Mengkai Li,Ming‐Cheng Luo ,Qunqun Hao,Xianming Chen,Daolin Fu

doi:10.1038/s41467-019-11872-9

Abstract

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production. Aegilops tauschii, one of the wheat progenitors, carries the YrAS2388 locus for resistance to Pst on chromosome 4DS. We reveal that YrAS2388 encodes a typical nucleotide oligomerization domain-like receptor (NLR). The Pst-resistant allele YrAS2388R has duplicated 3’ untranslated regions and is characterized by alternative splicing in the nucleotide-binding domain. Mutation of the YrAS2388R allele disrupts its resistance to Pst in synthetic hexaploid wheat; transgenic plants with YrAS2388R show resistance to eleven Pst races in common wheat and one race of P. striiformis f. sp. hordei in barley. The YrAS2388R allele occurs only in Ae. tauschii and the Ae. tauschii-derived synthetic wheat; it is absent in 100% (n = 461) of common wheat lines tested. The cloning of YrAS2388R will facilitate breeding for stripe rust resistance in wheat and other Triticeae species.

Highlights

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production
At the two-leaf seedling stage, CIae[9], PI511383, and/or PI511384 were resistant with infection types (IT) between 1 and 5 to nine Pst races (PSTv-3, PSTv-4, PSTv-11, PSTv-18, PSTv-37, PSTv-41, PSTv-51, PSTv-52, and PSTv-172), under low temperature (LT) and/or high temperature (HT) regimes (Table 1)
YrAS2388R has had limited use probably for two reasons: it is absent in common wheat; and it can be suppressed in hexaploid wheat

Summary

Introduction

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a global threat to wheat production. Wheat stripe rust, caused by Puccinia striiformis f. Tritici (Pst), is a global threat to wheat production. Planting wheat cultivars with adequate levels of resistance is the most practical and sustainable method to control stripe rust. ASR confers high levels of resistance to specific Pst races, but the underlying genes, such as Yr98 and Yr179, are often circumvented by the emergence of new virulent races. APR typically provides a partial level of resistance, but is more durable and is effective against all or a wider spectrum of Pst races than ASR. High-temperature adult-plant (HTAP) resistance is a major type of APR; HTAP typically provides durable and non-race-specific resistance to Pst[10]. Incorporating multiple ASR and HTAP genes appears to be an excellent strategy for maintaining sustainable resistance to wheat stripe rust[10]

Methods

Results

Conclusion