Abstract
BackgroundStripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. A genome-wide association study was conducted on 240 Chinese wheat cultivars and elite lines genotyped with the wheat 90 K single nucleotide polymorphism (SNP) arrays to decipher the genetic architecture of stripe rust resistance in Chinese germplasm.ResultsStripe rust resistance was evaluated at the adult plant stage in Pixian and Xindu in Sichuan province in the 2015–2016 cropping season, and in Wuhan in Hubei province in the 2013–2014, 2016–2017 and 2018–2019 cropping seasons. Twelve stable loci for stripe rust resistance were identified by GWAS using TASSEL and GAPIT software. These loci were distributed on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4B (3), 4D, 6D, and 7B and explained 3.6 to 10.3% of the phenotypic variation. Six of the loci corresponded with previously reported genes/QTLs, including Sr2/Yr30/Lr27, while the other six (QYr.hbaas-1BS, QYr.hbaas-2BL, QYr.hbaas-3AL, QYr.hbaas-4BL.3, QYr.hbaas-4DL, and QYr.hbaas-6DS) are probably novel. The results suggest high genetic diversity for stripe rust resistance in this population. The resistance alleles of QYr.hbaas-2AS, QYr.hbaas-3BS, QYr.hbaas-4DL, and QYr.hbaas-7BL were rare in the present panel, indicating their potential use in breeding for stripe rust resistance in China. Eleven penta-primer amplification refractory mutation system (PARMS) markers were developed from SNPs significantly associated with seven mapped QTLs. Twenty-seven genes were predicted for mapped QTLs. Six of them were considered as candidates for their high relative expression levels post-inoculation.ConclusionThe resistant germplasm, mapped QTLs, and PARMS markers developed in this study are resources for enhancing stripe rust resistance in wheat breeding.
Highlights
Stripe rust is a significant disease for bread wheat (Triticum aestivum L.) worldwide
Broad-sense heritability (H2) for maximum disease severity (MDS) was estimated to be 0.91, indicating less environmental variance than genotypic variance; this panel was suitable for further genetic analysis
High Pearson’s correlation coefficients (0.62–0.79) of stripe rust MDS were observed across five environments (Fig. 1)
Summary
Stripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. Tritici (Pst), is a significant disease of bread wheat (Triticum aestivum L.) worldwide. Genetic studies on stripe rust resistance are of great significance. 82 stripe rust resistance genes have been permanently designated [5], and many. Jia et al BMC Plant Biology (2020) 20:491 more have been given temporary names or quantitative trait locus (QTL) designations [6]. These genes were summarized in 2013 to more than 140 QTLs identified in various studies [7]. Out of the identified resistance genes, eight genes were isolated (Yr36 [8], Yr18 [9], Yr10 [3], Yr46 [10], Yr5/YrSP, Yr7 [11], Yr15 [12], and YrAS2388R [13])
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