An Analytical Investigation of in Vivo Mechanical References for Mechanobiological Experiments of Vascular Cells

Abstract

Blood vessels interact with their mechanical environments in a comprehensive way. Local mechanical stimuli outside the biological range play important roles in various human cardiovascular diseases. Although many mechanobiological studies of endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) in vitro have been reported in mimicking cellular dysfunctions, their quantitative correlations to the in vivo vascular conditions remain unclear. In order to interpret the stress-modulated dysfunctions of vascular cells and explore the key mechanical factors in vascular diseases, it is important to investigate the mechanical environments of vessel walls in vivo under various physiological conditions. Based on nonlinear continuum mechanics, we analyzed the variations of the mechanical stress, strain, and wall stiffness in human blood vessels at different blood pressures. We adopted nine middle-aged arteries located at different physiological sites for stress analysis including three aortas (ascending thoracic, descending thoracic, and abdominal), and five arterial branches (common iliac, femoropopliteal, subclavian, common carotid, and renal, and left anterior descending coronary artery). The femoropopliteal arteries aged from 11 to 70 years were also adopted for investigating the aging effects. It is found that 1) the vascular cells experience various mechanical stimuli along the arterial tree; 2) the intima and adventitia exhibit distinct variations in stress and strain during the femoropopliteal artery aging; and 3) the magnitude of wall stiffness seems to depend on the arterial locations rather than aging. Although it is reported that stress concentration usually occurs in intima causing EC dysfunctions, our results suggest that the adventitia is more likely to bear high stresses in middle-aged aortas and aged femoropopliteal arteries, triggering the vascular inflammation. We conclude that the mechanical niches of vascular cells strongly depend on the physiological site and aging process. The present results contribute to a better understanding of the mechanical environments in vessel walls, which could serve as a reference for studying the vascular cell mechano-transduction.