Abstract
BackgroundAs there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged (smf) or solid-state fermentation (ssf) using pomelo peel powder, an agro-forestry residue as carbon substrate. ResultsCultures grown in submerged fermentation produced a peak of exo-polygalacturonase activity as 6160 Ul-1 on the 4th day of culture as compared with 2410 Ul-1 on the 5th day of fermentation by solid-state fermentation. The enzyme yield coefficient (YE/X) is of higher value in smf vs. ssf system (YE/X = 1.05 × 103 vs. 0.622 × 103) indicating the more efficient product yield in smf as compared with ssf. The plots derived fromλ versusζ clearly demonstrate that the secondary product destruction is higher in smf than in ssf. ConclusionP. ostreatus performs much better in submerged fermentation as compared with solid-state fermentation in respect to exo-polygalacturonase production although ssf technique produced a more thermo-stable exo-polygalacturonase in crude extract, which is highly desirable in various industrial applications.
Highlights
As there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged or solid-state fermentation using pomelo peel powder, an agro-forestry residue as carbon substrate
Pomelo (Citrus maxima), a member of the family Rutaceae is a rich source of pectin and the yield of pure pectin obtained from this peel is 80.88% and it is rated as high methoxyl pectin (HMP) (DE = 92.75%) with a low viscosity [6]
It is evident that addition of sucrose (5 gl−1) in culture medium containing pomelo peel powder induced an enzyme production as induction ratio of 1.198 in solid-state fermentation, while a repression ratio of −0.133 was observed in culture filtrate enzyme production of submerged fermentation technique at the same initial level of sucrose in the culture medium
Summary
As there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged (smf) or solid-state fermentation (ssf) using pomelo peel powder, an agro-forestry residue as carbon substrate. Pomelo (Citrus maxima), a member of the family Rutaceae is a rich source of pectin and the yield of pure pectin obtained from this peel is 80.88% and it is rated as high methoxyl pectin (HMP) (DE = 92.75%) with a low viscosity [6] Both the fermentation systems, i.e., smf and ssf have been applied for the production of enzymes such as esterase, invertase, tannase, β-fructofuranosidase, and exo-pectinase, no comparative kinetic studies have been done to explain the differential behavior of micro-organisms for exo-polygalacturonase production in smf and ssf. This study has focused on the physico-chemical properties of crude exo-polygalacturonase, produced by the two kinds of fermentation techniques
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