Abstract
In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate (Pani-Pvs) films has been accomplished via the entrapment technique. Electropolymerization of aniline on the Pt surface of the Pt electrode was carried out at constant potential (0.75 V, vs. Ag/AgCl) using an electrochemical cell containing aniline and polyvinylsulphonate. Firstly, the optimum working conditions for preparing polyaniline-polyvinylsulfonate films were investigated. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and the optimum pH value was found to be 7.5. The storage stability and operational stability of the enzyme electrode were also studied. The results show that 75% of the response current was retained after 16 activity assays. The prepared glucose biosensor retained 80.6% of initial activity after 40 days when stored in 0.1 M phosphate buffer solution at 4 °C.
Highlights
Determination of glucose is essential due to its clinical and industrial importance
Most electrochemical glucose biosensors are based on the glucose oxidase (GOx) enzyme, which catalyzes the oxidation of glucose to gluconolactone which is hydrolyzed to gluconic acid and hydrogen peroxide
The parameters effecting to the performance of the biosensor were investigated
Summary
Determination of glucose is essential due to its clinical and industrial importance. The immobilization technique for localizing an enzyme at the surface of various electrodes plays a very important role in research on glucose biosensors. Conducting polymers are being widely used in biosensor applications because they provide stable and porous matrices for biocomponent immobilization and they facilitate the electron transfer process. The most widely used conducting polymers for immobilization of enzymes are polyaniline, polypyrrole, polythiophene etc. Most electrochemical glucose biosensors are based on the glucose oxidase (GOx) enzyme, which catalyzes the oxidation of glucose to gluconolactone which is hydrolyzed to gluconic acid and hydrogen peroxide. First the optimum working conditions to prepare the polyaniline-polyvinylsulphonate (Pani-Pvs) film were investigated. Pani-PVS film for determination of glucose via an entrapment procedure. The optimum working conditions of the biosensor with respect to the substrate concentrations, the pH and temperature were investigated
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