An alternative bioreactor concept for application of an isolated oxidoreductase for asymmetric ketone reduction

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In this paper an isolated NADH dependent ketone reductase has been used to synthesise ( S)-6-bromo-β-tetralol from 6-bromo-β-tetralone, together with commercially available formate dehydrogenase (FDH) as a recycle enzyme to produce preparative quantities of the product. Furthermore, initial experiments indicate potential for an alternative bioreactor concept via the use of a resin (XAD L-323) to bind the product (and residual substrate) of the conversion rather than the cofactors or enzymes, thus allowing a new method of recycle, potentially overcoming existing problems.