Abstract
This is the description of a method to obtain DAPI-banding in fishes' chromosomes by its denaturation using formamide. The slides are aged in an incubator and dehydrated in an ethanol series. The next step is the dena- turation in formamide 70% and staining with DAPI. The pattern obtained is mostly C-band like, but matches only partially with the C-band pattern usually found by Barium Hydroxide technique. The bands obtained also do not match with the band pattern by CMA 3 . These data and a review of literature bring the conclusion that the banding is related to the DNA base composition instead of being related to the chromosome structural changes resulting from a long denaturation process.
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