An activity of caspase-3 and cathepsin D at the different subtypes of ischemic stroke

Abstract

Aim of research – To define the dynamics of activity of caspase-3, cathepsin D, apoptosis of leukocytes at the different subtypes of ischemic stroke (IS) in an acute period.Methods. There were examined 232 patients in an acute period of ІS: 56 (24,1%)- with hemodynamic (HDS), 62 (2,.7%) – with atherothrombotic (АТS), 60 (25,9%) – with cardioembolic (CЕS) і 54 (23,3%) – with lacunar stroke (LS). There was defined the number of leukocytes at the stage of apoptosis (ANV+-cells), necrosis (PI+-cells), with an increased content of the active forms of oxygen (AFO+-cells) and with lowered mitochondrial potential (Mito+-cells), activity of caspase-3 and cathepsin D.Results. It was established that at all subtypes of IS mitochondrial dysfunction, apoptosis and necrosis of leukocytes are observed on the 1st day it were presented in increase of content of ANV+-, PI+-, АFO+- and Mito+-cells and were the mostly apparent at ATS. The highest activity of caspase-3 on the 1st day was noticed at LS it did not correlate with a number of cells at the stage of apoptosis and probably was connected with a predominant impact of caspase-3 on endothelium and with hyperpermeability of hematoencephalic barrier. In patients with ATS an activity of cathepsin D increased during the 1st week of disease that can indicate an activation of lysosomal way of activation of apoptosis that courses parallel to an apoptosis connected with mitochondrial dysfunction.Conclusions. The different ways of apoptotic cellular death that depends on subtype of stroke activate in an acute period of IS