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https://doi.org/10.1016/s0956-5663(97)00008-0
Copy DOIJournal: Biosensors and Bioelectronics | Publication Date: Jan 1, 1997 |
Citations: 43 |
Poly-( o-phenylenediamine was electropolymerized on the surface of an alcohol dehydrogenase-nicotimanide adenine dinucleotide-modified carbon paste electrode to yield an electrochemical biosensor for ethanol. Efficient electrocatalytic oxidation, at low applied potentials, of the enzymatically produced NADH by the conducting film provided a current that was dependent on ethanol concentration. The influence of various experimental variables was explored to optimize the analytical performance. Linear responses for ethanol in the range 3 × 10 −8 − 3 × 10 −6 M and a response time of 20 s were obtained. The trend in sensitivity towards different alcohols is in agreement with the known biospecificity of yeast ADH. Ethanol sensor was applied to the analysis of different alcohol beverages, resulting in excellent accuracy and precision.
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