Abstract
Background Previous studies have shown that Ampelopsin has an inhibitory effect on human tumors. However, the effect of Ampelopsin on renal cell carcinoma (RCC) is rarely reported. Therefore, this study aims to explain the role of Ampelopsin in RCC. Methods Different concentrations of Ampelopsin (0, 10, 25, 50, and 100 μM) were used to treat 786-O cells. Cell viability was detected by MTT assay, colony formation assay, and flow cytometry assay. Transwell assay and Wound healing assay were used to detect cell migration and invasion. Western blot analysis was applied to detect protein expression. Results Ampelopsin inhibited cell proliferation and induced apoptosis in RCC. And Ampelopsin can inhibit cell migration and invasion in RCC. All these results changed in a dose-dependent manner. Ampelopsin (100 uM) had the strongest inhibitory effect on cell viability and metastasis. In addition, Ampelopsin negatively regulated the PI3K/AKT signaling pathway in RCC cells. Moreover, Ampelopsin was only cytotoxic to RCC cells. Conclusion Ampelopsin inhibits cell viability and metastasis in RCC by negatively regulating the PI3K/AKT signaling pathway.
Highlights
Renal cell carcinoma (RCC) accounts for about 90% of renal malignancies, and the ratio of male to female is about 2 : 1.e age of onset was more than 40 years old, with a median age of 65 years [1]
Metastatic renal cell carcinoma (RCC) has a poor prognosis and is not sensitive to radiotherapy and chemotherapy. e 5-year survival rate of metastatic RCC is less than 10% [4]
There are no obvious symptoms in the early stage of RCC, and about 30% of patients are already metastatic RCC at the time of diagnosis [5]. erefore, it is very important to find effective drugs for the treatment of metastatic RCC
Summary
Renal cell carcinoma (RCC) accounts for about 90% of renal malignancies, and the ratio of male to female is about 2 : 1.e age of onset was more than 40 years old, with a median age of 65 years [1]. Erefore, it is very important to find effective drugs for the treatment of metastatic RCC. The effect of Ampelopsin on renal cell carcinoma (RCC) is rarely reported. Erefore, this study aims to explain the role of Ampelopsin in RCC. Ampelopsin inhibited cell proliferation and induced apoptosis in RCC. Ampelopsin can inhibit cell migration and invasion in RCC. All these results changed in a dose-dependent manner. Ampelopsin (100 uM) had the strongest inhibitory effect on cell viability and metastasis. Ampelopsin negatively regulated the PI3K/AKT signaling pathway in RCC cells. Ampelopsin was only cytotoxic to RCC cells. Ampelopsin inhibits cell viability and metastasis in RCC by negatively regulating the PI3K/ AKT signaling pathway
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