Abstract
The influence of tree age and the effect of growth regulators on the micropropagation of the carob (Ceratonia siliqua L.) from the axillary buds of mature trees have been described. Significant differences (P 3. The use of 0.5 mg/l BA and 0.2 mg/l IBA was the most favorable for shoots neoformation. The leafy shoots are propagated in MS medium with BA at a concentration of 1.5 mg/l. The addition of gibberellic acid at 0.2 mg/l in the culture medium allows a good elongation and development of the shoots of the carob. The effect of the age of the plant material used has shown that explants taken from mature carob trees have a low capacity for bud sprouting and shoot proliferation compared to those taken from juvenile trees. Rooting has been successful when the plant material used is taken from young trees on an MS medium containing 2 mg/IBA, with an average number of 3 to 4, roots 1 to 2 cm long, then for the adult material, no rooting was observed. Based on these tests, it appears that micropropagation of the carob from the axillary buds is feasible, but additional work must be done to root this recalcitrant material.
Highlights
Morocco has an important source of wealth in plant genetic resources, thanks to its geographical location and its pedoclimatic diversity
6-Benzylaminopurine GA3 (BA) stimulated buds with a percentage between 21% and 34.66%, as regards IBA and whatever the combination used, the use of IBA is always better than the use of BA alone (Figure 1), the results obtained in Table 1 showed that the combination of BA and IBA, increased the percentage of new shoots of axillary buds between 23% and 46%, the best combination is 0.5 mg/l of BA and 0.2 mg/l of IBA, which increases the number of newly formed shoots 2.28 ± 0.17 and allows a slight elongation of the stems 0.60 ± 0.06 cm (Table 1)
The axillary buds collected from trees of different ages were grown on Murashinge and Skoog (MS) media supplemented with BA (0.5 mg/l) and IBA (0.2 mg/l) and GA3 (0.2 mg/l), after four weeks, the results showed a significant difference in response between the different explants on the rate of bud sprouting, the number of shoots per bud and the size of the shoots
Summary
Morocco has an important source of wealth in plant genetic resources, thanks to its geographical location and its pedoclimatic diversity. Carob tree can be propagated by seed and vegetatively by stem cuttings, in populations resulting from seed propagation, the production is not uniform and the sex of the plants is not known until the appearance of the first flower buds [7] [8]. Numerous studies and tests, using juvenile and adult explants, have been carried out to improve the micro-propagation of the carob tree [9]-[18]. This study was carried out to investigate the influence of tree age, the effects of plant growth regulators on the in vitro propagation of mature C.siliqua trees. Experiments to evaluate the root formation of C. siliqua were tested
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
