Abstract
A high efficient four step protocol (callus initiation, regeneration, shoot elongation and rooting) for in vitro propagation of Dracaena sanderiana Sander ex Mast was developed. Callusing was achieved from nodal stem segment explants treated with various concentrations of ethylmethane sulphonate (EMS) on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 1.5 g m−3). A significant increase in callus induction percentage and biomass production was noticed from lower EMS treated lines (ET1 and ET2) comparatively to control and other (ET3, ET4 and ET5) lines. Calli of ET1 line showed high regeneration potential on MS medium with N 6-benzylaminopurine (BAP; 1.75 g m−3). Length of microshoots, which was reduced by EMS, restored by addition of gibberellic acid (GA3; 0.4 g m−3). A marked increase in rooting with increasing EMS concentration was noticed on MS medium fortified with 3-indolebutyric acid (IBA; 1.5 g m−3).
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