Abstract
Shoot organogenesis and plant regeneration were achieved on callus derived from leaf section and stem base explants of Quisqualis indica (Combretaceae). In vitro cultures were established using nodal segments obtained from mature field-grown shrubby plants. For the development of optimized protocol, different types and concentrations of plant growth regulators were used to induce adventitious shoot regeneration via callus from leaf section and one-node stem base explants obtained from in vitro regenerated micro shoots and direct field-grown newly flush-off shoots. The TDZ was considered to be the best among the cytokinins (6-benzyladenine (BA), 6-(?-?, dimethylallyamino purine) (2-iP) and thidiazuron (TDZ) added to the Murashige and Skoog’s medium (MS) for adventitious shoot productions. A combination of 1.0 mg/L TDZ and 0.5 mg/L GA3 was most effective in stimulating callus induction and adventitious shoot regeneration from the leaf section derived calli with an average of 6 shoots per callus explant and an average of 8 shoots per callus explant originated from one-node stem base explants. In vitro raised shoots were sub-cultured on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L GA3 for further shoot growth. Maximum rooting of in vitro regenerated shoots was obtained on MS medium supplemented with either 0.5 mg/L indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) individually or a combination of 0.5 mg/L IAA and 0.5 mg/L IBA. Plantlets raised in vitro were acclimatized and subsequently transferred to experimental field.
Highlights
Rangoon Creeper scientifically known as Quisqualis indica originated from South East Asia and occurs all over Africa, Philippines, Vietnam, Malaysia, India, Bangladesh and Thailand
A combination of 1.0 mg/L TDZ and 0.5 mg/L GA3 was most effective in stimulating callus induction and adventitious shoot regeneration from the leaf section derived calli with an average of 6 shoots per callus explant and an average of 8 shoots per callus explant originated from one-node stem base explants
The TDZ treatment was superior to that of 2-iP and BA in combination with either GA3 or naphthaleneacetic acid (NAA) in terms of inducing the formation of multiple shoots from organogenic callus derived from leaf section and one-node stem base explants of this ornamental shrubby climber Quisqualis indica
Summary
Rangoon Creeper scientifically known as Quisqualis indica originated from South East Asia and occurs all over Africa, Philippines, Vietnam, Malaysia, India, Bangladesh and Thailand It has bright colored fragrant flowers and is one of the most stunning ornamental of the family Combretaceae. The flowers contain high quantity of polyphenol that are believed to be strong antioxidants beneficial for human health [1,2,3] This species is known to have free radical scavenging activity and alleviating flatulent distension of abdomen like that of the medicinal properties of Terminalia chebula, T. belerica and Emblica officinalis [4,5,6]. The leaves, fruits and seeds of the plant have been used as anthelmintic for expelling round worms and thread worms [8,9,10] This ornamental shrub is conventionally propagated through seeds and cuttings. According to Lambardi and Rugini [11] propagation through seeds renders undesirable variation whereas shoot cuttings of many genotypes do not respond to root inducing medium
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
