Abstract
The goal of this study was to investigate the correlation between the potential nitrification (PN) rate and ammonia-oxidizing bacteria (AOB) in wetlands treated with swine wastewater. The wetlands showed high efficiency in the removal of NH4+, NO3−, and chemical oxygen demand (COD). Nitrification and denitrification occurred concurrently in the wetlands. The most probable number (MPN) value of AOB was highly related to NH4+ (R2=0.92, P<0.05) and NO3− concentrations (R2=0.96, P<0.05), but not significantly correlated with COD. The AOB Shannon–Weaver diversity index (H) and richness (S) which were calculated from digitalized profiles of denaturing gradient gel electrophoresis (DGGE) did not correlate with NH4+ and NO3− concentrations. The PN rates in detritus and soil samples highly correlated with NH4+ (R2=0.92, P<0.5). The phylogenetic trees based on 16S rRNA gene sequences and amino-acid-residue sequences of the amoA gene showed that parts of the AOB from the wetlands were affiliated with β-proteobacteria cluster 2, cluster 6a, cluster 7, and Nitrosospira sp. NSP65.
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