Abstract
Publisher Summary This chapter describes the mechanism of transport along with the genetic and regulatory interrelationships of multiple transport systems for an amino acid. The chapter focuses on LIV-I, which is a high-affinity branched chain amino acid transport system in Escherichia coli ( E.coli ). Some of the physiological and regulatory relationships of amino acid transporters are also presented, including a newly recognized global regulator, the leucine-responsive regulatory protein (Lrp). The LIV-binding protein (LIV-BP) bounds leucine, isoluecine, and valine, and it is a component of the LIV-I transport system. The leucine-specific binding protein (LS-BP) binds D- and L-leucine as a component of the LS system. The high-affinity branched chain amino acid transport system is regulated by both repression and transcription attenuation. Transcription attenuation control of LIV-I expression is identified genetically. High-affinity transport activity is altered by mutations in several genes involved in the formation of mature, charged leucyl-tRNA Leu . The cloning and sequence analysis of the LIV-I regulon provide a molecular framework that can account for the previous genetic results.
Published Version
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