Amino acid sequence determination of guuanyl-specific ribonuclease Sa from Streptomyces aureofaciens

Abstract

Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl-specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues ( M r 10 566). A 50% sequence homology of ribonuclease Sa to the guanyl-specific ribonuclease St from S. erythrew was found.