Abstract

The complete amino acid sequence of Penicillium chrysogenum 152A guanyl-specific RNase has been established using automated Edman degradation of two non-fractionated peptide mixtures produced by tryptic and staphylococcal protease digests of the protein. The RNase contains 102 amino acid residues: His 2, Arg 3, Asp 7, Asn 8, Thr 5, Ser 11, Glu 4, Gln 2, Pro 4, Gly 11, Ala 13, Cys 4, Val 8, Ile 3, Leu 3, Tyr 9, Phe 5 ( M r 10 747).

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