AMF Inoculation Can Enhance Yield of Transgenic Bt Maize and Its Control Efficiency Against Mythimna separata Especially Under Elevated CO2.

Long Wang,Xiaohui Wang,Changning Lv,Fanqi Gao,Likun Li,Tong Han,Fajun Chen

doi:10.3389/fpls.2021.655060

Long Wang, Xiaohui Wang + Show 5 more

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https://doi.org/10.3389/fpls.2021.655060

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Abstract

The promotion and application of transgenic Bt crops provides an approach for the prevention and control of target lepidopteran pests and effectively relieves the environmental pressure caused by the massive usage of chemical pesticides in fields. However, studies have shown that Bt crops will face a new risk due to a decrease in exogenous toxin content under elevated carbon dioxide (CO2) concentration, thus negatively affecting the ecological sustainability of Bt crops. Arbuscular mycorrhizal fungi (AMF) are important beneficial microorganisms that can effectively improve the nutrient status of host plants and are expected to relieve the ecological risk of Bt crops under increasing CO2 due to global climate change. In this study, the Bt maize and its parental line of non-transgenic Bt maize were selected and inoculated with a species of AMF (Funneliformis caledonium, synonyms: Glomus caledonium), in order to study the secondary defensive chemicals and yield of maize, and to explore the effects of F. caledonium inoculation on the growth, development, and reproduction of the pest Mythimna separata fed on Bt maize and non-Bt maize under ambient carbon dioxide concentration (aCO2) and elevated carbon dioxide concentration (eCO2). The results showed that eCO2 increased the AM fungal colonization, maize yield, and foliar contents of jasmonic acid (JA) and salicylic acid (SA), but decreased foliar Bt toxin content and Bt gene expression in Bt maize leaves. F. caledonium inoculation increased maize yield, foliar JA, SA contents, Bt toxin contents, and Bt gene expression in Bt maize leaves, and positively improved the growth, development, reproduction, and food utilization of the M. separata fed on non-Bt maize. However, F. caledonium inoculation was unfavorable for the fitness of M. separata fed on Bt maize, and the effect was intensified when combined with eCO2. It is indicated that F. caledonium inoculation had adverse effects on the production of non-Bt maize due to the high potential risk of population occurrence of M. separata, while it was just the opposite for Bt maize. Therefore, this study confirms that the AMF can increase the yield and promote the expression levels of its endogenous (JA, SA) and exogenous (Bt toxin) secondary defense substances of Bt maize under eCO2, and finally can enhance the insect resistance capacity of Bt crops, which will help ensure the sustainable utilization and safety of Bt crops under climate change.

Highlights

In recent years, many transgenic Bt crops, such as Bt maize and Bt cotton, have been grown around the world and have shown high resistance to specific target pests, mainly Lepidoptera insects (Wu et al, 2008; Liu et al, 2016)
Compared with ambient CO2 (aCO2), elevated CO2 (eCO2) significantly increased the arbuscular mycorrhizal fungi (AMF) colonization of non-Bt maize inoculated with F. caledonium in 2017 and 2018, that of Bt maize inoculated with F. caledonium in 2017, and that of Bt maize not inoculated in 2018 (P < 0.05; Figures 1A,C)
Compared with aCO2, eCO2 significantly increased the AMF-Phospholipid Fatty Acid (PLFA) content of Bt maize inoculated with F. caledonium in 2017 and 2018, and significantly increased the AMF-PLFA content of non-Bt maize inoculated with F. caledonium in 2018 and non-Bt maize not inoculated in 2017 (P < 0.05; Figures 1B,D)

Summary

Introduction

Many transgenic Bt crops, such as Bt maize and Bt cotton, have been grown around the world and have shown high resistance to specific target pests, mainly Lepidoptera insects (Wu et al, 2008; Liu et al, 2016). Bt crops have been used to control a wider range of pests, such as Helicoverpa armigera (Hübner), Heliothis virescens, and Mythimna separata Human activities, fossil fuel burning and land-use change, are rapidly increasing the level of carbon dioxide (CO2) in the atmosphere (Yu and Chen, 2019; Yao et al, 2020). It has been reported that the atmospheric CO2 concentration increased from 288 to 405 ppm from 1800 to 2018 (www.esrl.noaa.gov/gmd/ccgg/trends/). With the acceleration of industrialization, it is estimated that the concentration of CO2 in the atmosphere will increase from 800 to 1,000 ppm by the end of the twenty-first century (Pachauri and Reisinger, 2014)

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