Ameliorative Effects of Neurolytic Celiac Plexus Block on Stress and Inflammation in Rats with Partial Hepatectomy

J Li,P Zha,Qm Qiu,T Chen,Fx Li,Jx Che,Xh Wei,Sr Bai,Xd Tang

doi:10.4314/tjpr.v14i6.12

Abstract

Purpose: To investigate effects of neurolytic celiac plexus block (NCPB) on stress and inflammation in rats with partial hepatectomy (PH).Methods: A model of PH rat was established, and serum C-reactive protein (CRP); corticosterone (GC); adrenocorticotropin (ACTH); noradrenaline (NA); adrenalin (AD); aspartate transaminase (AST); alanine transaminase (ALT); as well as tumor necrosis factor-α (TNF-α); interleukin (IL)-1β and IL-6; high mobility group box1 (HMGB1); and nitric oxide (NO) concentrations in serum assessed after PH. Additionally, Western blotting was performed to determine the effect of NCPB on expressions of glucocorticoid receptors (GR), inhibitor of nuclear factor kappa B (IκB), p65, c-Jun and inducible nitric oxide synthase (iNOS) of PH rats, as well as assay effects of NCPB on nuclear translocation of GR, c- Jun and p65. DNA binding activities of nuclear factor kappa B (NF-κB) and activator protein 1 (AP-1) were also determined.Results: NCPB reduced AST and ALT (P < 0.05), decreased secretion of inflammatory cytokines and NO (P < 0.05), as well as decreased CRP, GC, ACTH, NA and AD after PH (p < 0.05). NCPB increased expressions of GR and IκB, but expressions of p65, c-Jun, and iNOS (p < 0.05). Additionally, NCPB increased nuclear translocation of GR (p < 0.01), but decreased nuclear translocation of p65 and c-Jun after PH (p < 0.05). Additionally, DNA binding activity of NF-κB and AP-1 was decreased by NCPB (p < 0.05).Conclusion: The results indicate that NCPB treatment can significantly inhibit stress and inflammation in PH rats.Keywords: Neurolytic celiac plexus block, Cytokine, Nuclear translocation, Partial hepatectomy, Stress, Inflammation

Highlights

Autonomic nervous system (ANS) plays important roles in stress and inflammatory reaction after severe trauma [1,2].The parasympathetic nervous system (PNS) can regulate the inflammatory reactions effectively through acetylcholine [3]
Results of our present investigation revealed that Neurolytic celiac plexus block (NCPB) is an effective way to relieve pain in clinic, and we found that the NCPB induced by 0.5 %
Neuroendocrine responses refers to the stress process started immediately after trauma, and a measurable neuroendocrine response is key for maintaining body homeostasis [16,17], systemic inflammatory response syndrome (SIRS) and immune function disorders can be induced by over-response

Summary

INTRODUCTION

Autonomic nervous system (ANS) plays important roles in stress and inflammatory reaction after severe trauma [1,2].The parasympathetic nervous system (PNS) can regulate the inflammatory reactions effectively through acetylcholine [3]. The solar plexus play crucial effects on regulation the functions of organ in epigastric region including liver, guts, pancreas, etc. Severe trauma can induce strong stress and inflammatory reactions, leading to over-activation of inflammatory cascade reaction, even the systemic inflammatory response syndrome (SIRS) [8,9]. It’s important to alleviate the damages of severe liver trauma and liver cancer surgery in order to suppress or control the levels of stress and inflammatory reaction. All other chemicals used in this study were of analytical reagent grade

Ethics statement

RESULTS

DISCUSSION