Abstract
The aim of this present study was to investigate the effect of chlorophyllin (CHL) on oxidative stress in Streptozotocine (STZ) induced diabetic mice. For the study, mice were divided into Group A: normal control, Group B: diabetic control, Group C: diabetic mice treated with the ascorbic acid, and Group D: diabetic mice treated with CHL. Levels of Reactive Oxygen Species (ROS), lipid peroxidation, protein carbonyl, superoxide dismutase (CuZn SOD &Mn-SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were examined in kidney and heart tissues of different experimental groups. Histological and ultrastructuralstudies were also carried out to evaluate any changes in tissues as well as sub-cellular organelles. ROS, lipid peroxidation, and protein carbonyl levels have been significantly decreased with concomitant increased of CuZn SOD, Mn-SOD, CAT, GPx, and GR activity in CHLtreated diabetic mice. The histological and ultrastructural studies showed that CHL attenuates the detrimental effect of oxidative stress and alleviated tissue injuries in STZ induced diabetic mice. These results suggested that CHL possesses antioxidative activity and has the potential to amelioratediabetes-associated oxidative stress in mice.
Highlights
Diabetes mellitus (DM) is a serious metabolic disorder with the key manifestation of prolonged hyperglycemia that is caused by either insulin deficiency or insulin inaction or both
Diabetic mice treated with ascorbic acid of Group C (0.59 ± 0.02nmol MDA/mg protein and 1.55 ± 0.14 in kidney and heart respectively) and CHL of Group D (0.59 ± 0.02 and 1.66 ± 0.12 in kidney and heart respectively) exhibited significant decrease of MDA level when compared to the diabetic mice of Group B (0.72 ± 0.03 and 2.12 ± 0.10 in kidney and heart respectively) as shown the result in figure 2
As shown the result in figure 4, Administration of ascorbic acid significantly increased the activity of CuZn SOD in diabetic mice of Group C as compared to the Group B (2.31 ± 0.10vs1.81 ± 0.09 in kidney and 1.85 ± 0.10vs 0.97 ± 0.11 in heart)
Summary
Diabetes mellitus (DM) is a serious metabolic disorder with the key manifestation of prolonged hyperglycemia that is caused by either insulin deficiency or insulin inaction or both. The prolonged hyperglycemic condition during DM stimulates the production of more ROS through inducing several mechanisms such as glucose oxidation, polyol pathway, protein kinase C activation, and advanced glycation end product formation [1,2]. Mammalian cells are equipped with both enzymic and non-enzymic antioxidant defense systems to minimize the cellular damage caused by ROS. The relative overload of ROS and diminution of antioxidant defense systems activity in DM contributes to the generation of more oxidative stress that plays a crucial role in the pathogenesis and complications of DM [3,4]. CHL possess therapeutic importance due to its antimutagenic and anticarcinogenic [7], antioxidative [8], and antihyperglycemic effects
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call