Amelioration of Rifampicin and Isoniazid Induced Liver Oxidative Damage and Inflammation Response by Propolis Extracts in Rodent Model

Abstract

Liver diseases pose a serious threat to human health and the conventional drugs available for the treatment of this life threatening condition often causes inadequate results. Propolis has been used from centuries traditionally for their hepatoprotective effect. We wanted to assess the liver protective activity of Propolis. The aim of the present study was to investigate the liver protective effect of hydro-alcoholic & aqueous extracts of Propolis against isoniazid and rifampicin (INH-RIF) induced oxidative damage and inflammation response in Wistar rats. Two different extracts, hydro-alcoholic (KPHa) & aqueous (KPAq) extracts of Propolis (400 mg/kg b.wt. p.o.) were administered orally daily for 14 days to INH-RIF induced rats. The liver protective effect was assessed by hepatic enzyme markers, in-vivo antioxidant, histopathological and immunohistochemistry studies. The study group was compared with the control group by one-way ANOVA, followed by Bonferoni’s test. A p-value of <0.01 was considered significant. Hepatic cellular injury was initiated by administration of INH-RIF combination (100 mg/kg each) intraperitoneal (i.p.) injection for 14 days. Administration of both, KPHa & KPAq, extracts at a dose of 400 mg/kg b.wt, p.o markedly controlled all modulating oxidative, hepatic damage markers and resulted in a significant decrease of INH-RIF induced damage in liver. Our current study demonstrate that the KPHa & KPAq extracts (from Kashmir propolis) were liver protective agents against INH-RIF induced hepatic injury.