Amelioration of Obesity, Glucose Intolerance, and Oxidative Stress in High-Fat Diet and Low-Dose Streptozotocin-Induced Diabetic Rats by Combination Consisting of “Curcumin with Piperine and Quercetin”

Ginpreet Kaur,Meena C

doi:10.5402/2012/957283

Abstract

Curcumin is an important nutraceutical that has enormous potential for a variety of diseases, but the medicinal properties of curcumin cannot be utilized due to its low in vivo bioavailability. Therefore, in view of the foregoing, there is an extensive need for combinatorial extract “curcumin with piperine and quercetin” which may enhance bioavailability of oral curcumin by inhibiting the enzymes responsible for the metabolism of curcumin. Thus, the present study investigated the effect of combinatorial extract of curcumin on obesity, glucose intolerance, and oxidative stress in high fat diet and low-dose streptozotocin-induced rats. Oral administration of combinatorial extract for 28 days significantly (P < 0.05) reduced PGL (64.84%), PTG (88.94%), LDL (26.38%) and PTC (50.23%) levels, respectively and improved glucose tolerance (P < 0.05) significantly to exogenously administered glucose (2 g/kg) at 60, 90, and 120 min interval on OGTT. The results for antioxidant potential indicate that at 100 mg/kg dose of combinatorial extract of curcumin significantly prevented the high-fat diet and low-dose streptozotocin-induced changes in the oxidative stress parameters (P < 0.01) which supports popular medicinal uses of this combinatorial extract as antihyperglycemic and hypolipidemic and is likely to bring this promising natural product to the forefront of therapeutic agents in the in the treatment of “metabolic syndrome”.

Highlights

Metabolic syndrome, a concurrence of disorders including obesity, insulin resistance, dyslipidemia, and hypertension, has gained importance because of its association with subsequent development of cardiovascular disease and type 2 diabetes [1]
The present study demonstrates that the rats fed with highfat diet combined with low-dose streptozotocin resulted in significant elevation of plasma glucose as evident from elevated glycemic levels at 30, 60, 90, and 120 min after glucose challenge, compared with the vehicle-treated normal pellet diet (NPD) control group (P < 0.05) and exhibited the characteristic features of obesity and glucose intolerance namely increased body weight, hypertriglyceridemia, hypercholesterolemia and impaired glucose tolerance compared with NPD fed control groups
In the present study, we have isolated curcumin, piperine, and quercetin from Curcuma longa Linn, Piper nigrum, and Allium cepa, respectively and tested this combination consisting of curcumin with piperine and quercetin (CPQ) compounds to attenuate metabolic changes associated with high-fat diet and low-dose streptozotocin-induced rats

Summary

Introduction

A concurrence of disorders including obesity, insulin resistance, dyslipidemia, and hypertension, has gained importance because of its association with subsequent development of cardiovascular disease and type 2 diabetes [1]. In view of the foregoing, there is an extensive need for such combination of curcumin with piperine and quercetin as piperine is an alkaloid found naturally in plants as Piper nigrum L, commonly known as black pepper is established as a bioavailability enhancer of drugs. It is an inhibitor of glucuronidation metabolism increases bioavailability (serum concentration) of oral curcumin [14], and if a second bioavailability enhancer such as quercetin is added, an increase in absorption is possible to a greater extent as it aids in the inhibition of metabolic conversion of curcumin. In addition the antiobesity effect of combinatorial extract of curcumin on diet-induced changes in body weight, plasma glucose, triglyceride cholesterol, and low-density lipoprotein (LDL) were determined

Methods

Results

Discussion

Conclusion