Abstract
A new method combining atomic force (AFM) and confocal laser scanning microscopy (CLSM) and permitting not only to obtain information about the surface topography of nanostructures in the cell nucleus, but to simultaneously identify their molecular composition by using fluorescent dyes and treatment with specific enzymes, is proposed. The content of the vegetative nuclei of the ciliate Paramecium caudatum stained with DAPI for revealing DNA and fluorescently labeled phalloidin specifically binding fibrillar actin was been sequentially studied using CLSM and AFM and by successive analysis with AFM after RNAse and Proteinase K treatments. The proposed method allows one to characterize the surface topography of chromatin and RNP-complexes and reveal nanostructures containing nuclear actin which cannot be visualized using CLSM only.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
