Altered adhesion of dental sealant to tooth enamel microscopically modified by Er:YAG laser irradiation: An in vitro study.

Abstract

Er:YAG laser has become optional for enamel and dentin preparation for a decade. However, it is unclear if Er:YAG laser is acceptable for enamel conditioning prior to an application of a pit-and-fissure sealant. This in vitro study thus aimed to investigate shear bond strength of a sealant to enamel etched with Er:YAG laser, as well as to demonstrate an alteration of enamel surface after the laser was applied. One hundred and twenty extracted human premolars were divided into 4 groups (N = 30 per group) in which the enamel surfaces were treated with 1) 37% phosphoric acid, 2) Er:YAG laser 80 mJ/pulse, 2 Hz, 3) Er:YAG laser 120 mJ/pulse, 10 Hz, and 4) Er:YAG laser 140 mJ/pulse, 2 Hz. Prior to sealant application. Shear bond strength was determined by using a universal testing machine. Statistical analysis was performed using One-way ANOVA. Modification of enamel surface after laser ablation was also investigated using scanning electron microscope. Phosphoric acid-etched enamel yielded the highest shear bond strength when bonded with a sealant. Ablation of enamel with Er:YAG laser did not significantly improve sealant bonding since it demonstrated lower shear bond strength, compared to acid-etched enamel. Despite the presence of cracks after Er:YAG laser application, dissolution of enamel substances was limited. Our study has shown a reduced shear bond strength of dental sealant to an Er:YAG laser-irradiated enamel, compared with phosphoric acid-etched enamel.