Abstract
In the present study silver nanoparticles fabricated by using leaf extract of Alpinia calcarata. We have also studied the effect of various experimental parameters viz., metal ion concentration, pH and incubation period on nanoparticle biosynthesis. Results of optimization showed that metal ion concentration of 1.5 mM, alkaline pH and incubation period of 12 h were the optimum conditions for metal nanoparticle biosynthesis. Synthesized silver nanoparticles were characterized by UV–Visible spectroscopy, Dynamic light scattering (DLS), Zeta potential analysis, Fourier transform infrared spectroscopy (FTIR), Inductively coupled plasma-optical emission spectrometry (ICP-OES), Transmission electron microscopy (TEM) and X-ray diffraction analysis (XRD). The UV–visible spectrum shows a sharp peak at 420 nm which was due to the surface plasmon resonance of the silver nanoparticles. Effect of several phytochemicals present in A. calcarata, on synthesis of silver nanoparticles was studied by Fourier transform infrared spectroscopy. The results indicate that the flavonoids, phytosterol, quinones and phenolic compounds present in the plant extract plays a major role in formation of silver nanoparticles in their respective ions in solution. Results of TEM and XRD analysis showed that synthesized silver nanoparticles were mostly spherical in shape with an average diameter of 27.2 ± 0.2.5 nm and highly crystalline in nature. Moreover the synthesized silver nanoparticles were also evaluated for their potential antibacterial and antioxidant activities. It showed good antibacterial activity as well as antioxidant activity. Thus the obtained result provides a scientific support that leaf extract of A. calcarata can be used efficiently in the production of potential bioactive silver nanoparticles with several pharmaceutical applications.
Highlights
Fabrication methods for the synthesis of metal nanoparticles using green chemistry principle have gained significant attention in the past decades
3 Results and discussion In the present investigation we have demonstrated the biosynthesis of silver nanoparticles using leaf extracts of A. calcarata
The aqueous A. calcarata leaf extract consist a high amount of flavonoids and phenolic compounds suggesting that these biomolecules are the key components for synthesis of these silver nanoparticles
Summary
Fabrication methods for the synthesis of metal nanoparticles using green chemistry principle have gained significant attention in the past decades. This is mainly due to there is no requirement of toxic chemicals for synthesis protocol. Metal nanoparticles are gaining the interest of scientist for the novel methods of synthesis because they exhibit unique physicochemical properties including optical catalytic, magnetic, electronic and antimicrobial properties [2]. They have a high surface area and a high fraction of atoms. It has been found that a highly reactive metal nanoparticle shows excellent antimicrobial activities against bacteria [6] Silver nanoparticles have wide applications in medicines including skin creams and ointments containing silver to inhibit infection of burns and wounds and medical devices
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