Abstract
Blood flow to skeletal muscle is regulated in part by stimulation of vascular adrenergic receptors (ARs), which are expressed in several subtypes. The species and tissue distribution of these receptor subtypes has not been thoroughly examined. We sought to examine the alpha-1 (α-1) AR subtype distribution in mouse and rat mesenteric arteries. Using third-order mesenteric arteries from male Wistar rats and C57BL/6J mice, isometric force measurements were taken using a wire myograph. Arteries were exposed to increasing concentrations of the non-selective α-1 AR agonist phenylephrine (PE). The α-1A selective antagonist RS100329 elicited a potent inhibition of contractility in both rat and mouse tissue, while the α-1D selective antagonist BMY7378 had little to no effect on the contractile response to PE. These data suggest that, although there is not an apparent species dependent change in receptor subtype distribution, α-1A is the predominant α-1 AR subtype expressed in mesenteric tissue. This work was supported by the British Heart Foundation and the Wellcome Trust.
Published Version
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