Allogenous Freeze-Dried Platelet-Rich Plasma Promotes Peripheral Myelin Repair in Chronic Constriction Nerve Injury

Abstract

Background: Neuropathic pain is a chronic pain condition that refers to all pain that begins or is caused by a primary lesion or dysfunction or a temporary disorder in the peripheral or central nervous system (CNS). Neuropathic pain is caused by damage or injury to nerves that transfer information to the brain and spinal cord from the skin, muscles and other body parts. Platelet concentrates such as Platelet Rich Plasma (PRP) have been proven very useful for tissue regeneration since they contain high Growth Factors. PRP is tested for nerve injury to determine the potential of PRP in improving nerve. Objective: The purpose of this study is to prove the role of PRP in increasing the thickness of myelin in the process of repairing nerves on days 14 and 21. Methods: This study used three-month-old Rattus novergicus as experimental animals. Experimental animals were randomly divided into 7 groups, each consisting of six rats. On day 14 and 21 after treatment, rats were sacrificed. The examination of myelin thickness was obtained from Osmium Tetraoxide-Toluidine blue staining. Analysis of the data was performed with ANOVA test. Results: The differentiation capability of FD-PRP towards the sciatic nerve was better in the single dose PRP at 21 days, as indicated by an increase in axon remyelinization compared with differentiation in the chronic ligation group. The myelin thicknes was significantly increased (p 0.000 < 0.05) in FD-PRP groups compared to ligation group at day 21, whereas myelin thicknes no significantly increased (p < 0.05) in FD-PP groups compared to control group at day 21. Conclusion: PRP can increase axon remyelinization in neuroregeneration. Administration of PRP Single doses for 21 days show the most effective dose for neuroregeneration.