Alleviation of Cd-polluted paddy soils through Si fertilizer application and its effects on the soil microbial community

Abstract

In this study, the effects of slag-based Si fertilizers on Cd-polluted paddy soils, soil microbial diversity, and functional properties were evaluated through a long-term field experiment conducted in a double-rice cropping system in southern China. The results showed that soil pH significantly increased from 5.15 to 6.13 after seven years of Si fertilization. Cd accumulation in both the soil and rice plants were significantly decreased for all the Si fertilizers treatments. Treatments using Si fertilizer in powder form exhibited the best alleviation effects, where soil available Cd decreased from 0.50 mg kg−1 to 0.43 mg kg−1, and Cd accumulation in rice roots, straw, and grains decreased by 32.2 %, 57.2 %, and 45.5 %, respectively, than that in the control. Following Si application, the soil microbial richness and Shannon diversity increased from 6731 to 7549 and 7.12 to 7.28, respectively. Proteobacteria, Nitrospirae, and Gemmatimonadetes, were significantly enriched in the Si-treated samples, whereas Verrucomicrobia, Chlamydiia, Ktedonobacteria and Candidatus_Saccharibacteria exhibited opposite patterns. Bioinformatics analysis using phylogenetic investigation of communities by reconstruction of unobserved states tools revealed that the varied microbial community induced functional adaption of soil microorganisms involved in metabolism, genetic information processing, cellular processes, and environmental information processing. The soil pH, NH4-N, and available Cd and Si contents were the key factors that best explained the variations in bacterial community composition among different treatments. Slag-based Si fertilizers are effective for Cd detoxication and can benefit the growth of rice plants throng the regulation of soil microorganisms.