Alleviation of cadmium phytotoxicity by magnesium in Japanese mustard spinach

Abstract

To clarify the mechanism of Magnesium (Mg) in alleviating cadmium (Cd) phytotoxicity, Japanese mustard spinach (Brassica rapa L. var. pervirdis) was grown for 10 days after treatment in hydroponics in a growth chamber under natural light. The treatments were: (1) nutrient solution alone (Control), (2) 10 mmol L−1 Mg (High-Mg), (3) 2.5 µmol L−1 Cd (Cd-toxic), (4) 2.5 µmol L−1 Cd plus 10 mmol L−1 Mg (Mg-alleviated). The Cd-toxic treatment showed substantial growth retardation and chlorosis of young leaves, such symptoms were not observed in Mg-alleviated plants. Magnesium-alleviated plants showed higher shoot growth, more than twofold, and decreased shoot Cd concentration, approximately 40%, compared with Cd-toxic plants. This increase in shoot growth and simultaneous decrease in shoot Cd concentration may explain the alleviation of Cd toxicity with Mg in Japanese mustard spinach. In Cd-toxic plants, concentrations of K in shoots and Zn in both shoots and roots increased compared with the other three treatments. Concentrations and accumulations of Fe and Mn in shoots decreased significantly in the Cd-treated (Cd-toxic and Mg-alleviated) plants compared with the control and High-Mg plants. Thus, the application of high amounts of Mg in the nutrient solution can alleviate Cd toxicity in plants.